Intervention Studies for the metabolite Sulforaphane-N-acetyl-L-cysteine with food phytochemical Glucoraphanin

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	Food Phytochemical		Metabolite	Species	Biofluids	Origin	Dietary Source Description	Dietary Source Classification	Total Number of Subjects	Description of Population	Ingested Amount	Single/Multiple Dose	Duration of the Study	Tmax (h)	Cmax (nM)	AUC (nM/h)	Time covered by AUC (h)	t 1/2 (h)	% of 24h Urinary Excretion	Excreted Amount & Time Interval	Publications

	Food Phytochemical		Metabolite	Species	Biofluids	Origin	Dietary Source Description	Dietary Source Classification	Total Number of Subjects	Description of Population	Ingested Amount	Single/Multiple Dose	Duration of the Study	Tmax (h)	Cmax (nM)	AUC (nM/h)	Time covered by AUC (h)	t 1/2 (h)	% of 24h Urinary Excretion	Excreted Amount & Time Interval	Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Urine	Host metabolism	Broccoli sprouts (20 g) containing 64 µmol of glucoraphanin	Food	14	Healthy women; age= 27-36 Y	20g of broccoli sproutscontaining 64 µmol of glucoraphanin	Multiple	7 days								Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Urine	Host metabolism	Broccoli cooked sous vide in boiling water at 100 °C for 20 min (inactivated myrosinase), then pureed with 100 ml water (1.06 ± 0.18 μmol g−1 dry weight)	Food	12	Healthy, Age=18- 64 Y	200 g	Single	24 h							9.8±5,1 µmol (0-24h)	Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Urine	Host metabolism	Broccoli cooked sous vide in boiling water at 100 °C for 20 min (inactivated myrosinase), then pureed with 100 ml water and 1 g brown powdered mustard seed added (8.58 ± 0.33 μmol g−1 dry weight)	Food	12	Healthy, Age=18- 64 Y	200 g	Single	24 h							44.7±33,9 µmol (0-24h)	Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Plasma; Urine	Host metabolism	Broccoli (200g) with 20 g of raw daikon radish	Food	18	Healthy; 8M; 10W; Age=37–65 years; BMI = 19-37 kg/m²	200 g	Multiple	17 days once a day	1-3							Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Plasma; Urine	Host metabolism	200 g of uncooked broccoli florets	Food	6	Healthy; Age=28-67 Y; BMI= 25.3-28.1Kg/m²	200 g uncooked broccoli florets	Single									Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Plasma	Host metabolism	Myrosinase-activated broccoli seed extract (BroccoMax®). Blood samples were taken at 0, 0.5, 1, 2, 4 and 8 hours.	Extract	6	Healthy; F; Age= 18-35Y	32 mg sulforaphane	Single		2 (SEM)	66	315,5	0-8h				Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Plasma	Host metabolism	Myrosinase-non activated broccoli seed extract (BroccoMax®). Blood samples were taken at 0, 0.5, 1, 2, 4 and 8 hours.	Extract	6	Healthy; F; Age= 18-35Y	32 mg sulforaphane	Single		2 (SEM)	48	195,5	0-8h				Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Plasma; Urine	Host metabolism	200 g of blanched and frozen broccoli (100g in breakfast and 100g in the dinner) ( 147.6μmol ofglucoraphanin and 3.6μmol of glucoerucin).	Food	18	Healthy;9M;8F;Age=40-70Y	200g (the day before the study treated group eat only 100g and the day of the study all volunteers treated and non-treated eat 200g)	Multiple	17 days			4	24h		38		Publications
	Glucoraphanin		Sulforaphane-N-acetyl-L-cysteine	Human	Urine	Host metabolism	The treatment were applied during 10 weeks (70 days) and samples were taken on day 0 and 70 and after the treatment on day 90 and 160)	Food	40	Obese; Age= 35-55 Y; BMI= 24.9- 29.9 kg/m²	30 g per day	Multiple	70 days							0.193 µM (day 0); 2.301 µM(day 70); 0.023 µM (day 90); 0.094 µM (day 160)±0-0.41 µM (day 0); 1.85-2.75 µM(day 70); 0.01-0.04 µM (day 90); 0.0-0.19 µM (day 160) (CI)	Publications

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